Protocol for controlled gas-phase oxygen In-leakage in anaerobic bioreactors
Shota Shimokawara, Toshinari Maeda · 2026
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Description
Shota Shimokawara and Toshinari Maeda publish a protocol for leaking oxygen into oxygen-free bioreactors on purpose. Microbes that thrive without air are sensitive to traces of it, yet stray oxygen often sneaks into gas lines, and researchers need a controlled way to study the effect. Their method blends gases with precision flow controllers so the inlet oxygen can be set anywhere from zero to ten thousand parts per million.
Because the dose is fixed at the inlet, the exposure stays steady no matter how much oxygen the culture consumes inside. A gas chromatograph at the outlet confirms what the microbes actually met. The protocol supports both sudden step changes and long, constant exposures. The authors offer it as a shared standard for probing how trace oxygen shapes continuous anaerobic cultures, from gut microbes to gas-fermenting systems.
Catalog record
| CREATOR | Shota Shimokawara, Toshinari Maeda |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 17 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |