Transmission electron microscopy of cultured cells: Optimized protocols for ultrastructural studies and post-embedding immunogold labeling
Anna Richert, Robert Lenartowski, Jolanta Nowak, Piotr Wasąg, Marta Lenartowska · 2026
Read Transmission electron microscopy of cultured cells: Optimized protocols for ultrastructural studies and post-embedding immunogold labeling by Anna Richert, Robert Lenartowski, Jolanta Nowak, Piotr Wasąg, Marta Lenartowska (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13326743.txt.
Description
Anna Richert and co-authors set out tuned lab protocols for electron microscopy of cultured cells in a 2026 methods paper. Pinpointing proteins inside cells at nanometer scale guides work on gene activity, signaling, and cell traffic.
Their post-embedding immunogold routine labels one or more proteins in cells grown on plastic coverslips, whether the cells stick or float. Resin embedding is arranged so thin slices cut cleanly and collect well. The routine balances keeping cell structure crisp against keeping target sites readable by antibodies. A companion procedure covers plain ultrastructure study when labeling fails. Protein traits and cell compartment still sway the best settings.
Catalog record
| CREATOR | Anna Richert, Robert Lenartowski, Jolanta Nowak, Piotr Wasąg, Marta Lenartowska |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 43910 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |