Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics
Mengyang Jacky Li, Jinghui Wang, Mikolaj Walczak, Yuqing Qiu, Colleen Russell, Miroslaw Janowski, Piotr Walczak, Yajie Liang, Tian-Ming Fu, Brice Bathellier, Tian-Ming Fu, Mengyang Jacky Li, Jinghui Wang, Mikolaj Walczak, Yuqing Qiu, Colleen Russell, Miroslaw Janowski, Piotr Walczak, Yajie Liang · 2026
Read Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics by Mengyang Jacky Li, Jinghui Wang, Mikolaj Walczak, Yuqing Qiu, Colleen Russell, Miroslaw Janowski, Piotr Walczak, Yajie Liang, Tian-Ming Fu, Brice Bathellier, Tian-Ming Fu, Mengyang Jacky Li, Jinghui Wang, Mikolaj Walczak, Yuqing Qiu, Colleen Russell, Miroslaw Janowski, Piotr Walczak, Yajie Liang (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13282114.txt.
Description
Mengyang Jacky Li and co-authors describe a 2026 microscopy method for filming brain activity in three dimensions at high speed. Their tool uses a special light beam, called a Bessel beam, stretched along its axis. That shape turns slow three-dimensional scans into fast flat frame scans under a fluorescence microscope, which makes glowing cells visible. The setup is compact and low cost, and its focus can be tuned without moving its centre.
The team used the microscope to map blood flow in the brains of normal mice and mice after stroke. They paired it with light-based stimulation to chart links between nerve cells in living tissue. They also tracked immune cells in the brain after single-cell laser damage, watching varied responses over time and space. The authors say the speed, depth, and flexibility open new ways to study how brain cells, blood vessels, and immune cells interact.
Catalog record
| CREATOR | Mengyang Jacky Li, Jinghui Wang, Mikolaj Walczak, Yuqing Qiu, Colleen Russell, Miroslaw Janowski, Piotr Walczak, Yajie Liang, Tian-Ming Fu, Brice Bathellier, Tian-Ming Fu, Mengyang Jacky Li, Jinghui Wang, Mikolaj Walczak, Yuqing Qiu, Colleen Russell, Miroslaw Janowski, Piotr Walczak, Yajie Liang |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 115420 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |