Computer‐Aided Sperm Analysis Protocol for Evaluating Sperm Motility in Japanese Medaka

Miwa Kuroyanagi, Satoshi Ansai, Keigo Okamoto, Ai Kato, Touko Yamazaki, Yasuhiro Kamei, Eiji Watanabe, Kiyoshi Naruse, Yukiko Ogino · 2026

Read Computer‐Aided Sperm Analysis Protocol for Evaluating Sperm Motility in Japanese Medaka by Miwa Kuroyanagi, Satoshi Ansai, Keigo Okamoto, Ai Kato, Touko Yamazaki, Yasuhiro Kamei, Eiji Watanabe, Kiyoshi Naruse, Yukiko Ogino (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13253179.txt.

Description

Miwa Kuroyanagi and co-authors give a standard protocol for measuring sperm motility in Japanese medaka fish in a 2026 paper. Changes in sperm movement can flag reproductive harm from chemicals in the environment or from gene changes. Yet motility is very sensitive to salt balance, timing, and other outer factors, so readings often vary between labs.

Their protocol details sperm collection, activation, and computer aided measurement with a named analysis system. It focuses on how the share of moving sperm changes after activation and on effects of freezing. Movement and speed fell over time, and freezing sped the fall. Early measurement after activation is urged for fair comparisons.

Catalog record

CREATORMiwa Kuroyanagi, Satoshi Ansai, Keigo Okamoto, Ai Kato, Touko Yamazaki, Yasuhiro Kamei, Eiji Watanabe, Kiyoshi Naruse, Yukiko Ogino
YEAR2026
COLLECTIONPAPERS
FORMATTXT
FILE SIZE48 KB
VERSION1.0
LICENSECC BY 4.0
RIGHTSCC BY
SOURCEEurope PMC

Source: https://europepmc.org/articles/PMC13253179

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