Protocol for quantifying coccidian parasite mechanics and rupture force using advanced micromanipulation-based piercing
Jana El Husseiny, Pierre-Henri Puech, Aurélien Dumètre, Julien Husson · 2025
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Description
SummaryCoccidian parasites can rely on their mechanical properties to infect their hosts. Here, we detail a microindentation-based technique for quantifying the wall mechanics of coccidian oocysts. We describe steps for micropipette fabrication, calibration of flexible microindenters, system assembly, and quantifying indentation and rupture forces. We then present procedures for automated data analysis using open-source software and programming languages.
Catalog record
| CREATOR | Jana El Husseiny, Pierre-Henri Puech, Aurélien Dumètre, Julien Husson |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 73 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |