PacBio Sequencing and Its Applications
Rhoads A, Au KF. · 2015
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Description
Rhoads and Au reviewed PacBio sequencing and its applications in 2015. PacBio's single-molecule, real-time method reads one DNA molecule as it is copied. It offers much longer reads than the second-generation technologies that came before it.
Long reads suit problems that short reads cannot solve, such as spanning repeats and assembling genomes without gaps. The review covers uses in genome, transcriptome, and epigenetics research, including direct detection of chemical marks on DNA. Highly contiguous assemblies from long reads changed what genome projects could finish. The paper maps where this technology helps most and where its costs and errors still bind.
Catalog record
| CREATOR | Rhoads A, Au KF. |
|---|---|
| YEAR | 2015 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 49 KB |
| LICENSE | Creative Commons Attribution License |
| RIGHTS | CC BY |
| SOURCE | PubMed Central |