Multiplexed CRISPRi reveals a transcriptional switch between KLF activators and repressors in the maturing neocortex
Ryan W Kirk, Liwei Sun, Ruixuan Xiao, Erin A Clark, Sacha B Nelson, Joshua Corbin, Ryan Kirk, Liwei Sun, Ruixuan Xiao, Erin A Clark, Sacha B Nelson · 2026
Read Multiplexed CRISPRi reveals a transcriptional switch between KLF activators and repressors in the maturing neocortex by Ryan W Kirk, Liwei Sun, Ruixuan Xiao, Erin A Clark, Sacha B Nelson, Joshua Corbin, Ryan Kirk, Liwei Sun, Ruixuan Xiao, Erin A Clark, Sacha B Nelson (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13682713.txt.
Description
The authors study how young brain cells in mice switch off growth genes as the cortex matures after birth. By joining maps of open DNA with gene activity data from two cell types, they pointed to a family of gene switches named KLF. A method that quiets many genes at once let them test the family despite backup copies.
Early on, two family members turn target genes on; later, two others turn the same genes off. The targets help build the cell skeleton, and the switch may end the growth of long fibers. That shift could help move the young brain from wiring growth to fine-tuning links.
Catalog record
| CREATOR | Ryan W Kirk, Liwei Sun, Ruixuan Xiao, Erin A Clark, Sacha B Nelson, Joshua Corbin, Ryan Kirk, Liwei Sun, Ruixuan Xiao, Erin A Clark, Sacha B Nelson |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 177 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |