Methylome profiling of SetDB1-deficient ESCs reveals coordinated epigenetic cross-talk during pluripotency
Nick G.P. Bovee, Ehsan Habibi, Zicong Liu, Stefan H.A. Hoogland, Siebren Frölich, Daniëlle Diepenbroek, Kim C.M. Bosman, Julia Arand, Irina A. Maksakova, Anouk Klein Kranenbarg, Jörn Walter, Arie B. Brinkman, Klaas W. Mulder, Joop H. Jansen, Michiel Vermeulen, Matthew Lorincz, Hendrik G. Stunnenberg · 2026
Read Methylome profiling of SetDB1-deficient ESCs reveals coordinated epigenetic cross-talk during pluripotency by Nick G.P. Bovee, Ehsan Habibi, Zicong Liu, Stefan H.A. Hoogland, Siebren Frölich, Daniëlle Diepenbroek, Kim C.M. Bosman, Julia Arand, Irina A. Maksakova, Anouk Klein Kranenbarg, Jörn Walter, Arie B. Brinkman, Klaas W. Mulder, Joop H. Jansen, Michiel Vermeulen, Matthew Lorincz, Hendrik G. Stunnenberg (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13681314.txt.
Description
Nick Bovee and a large team mapped in 2026 how DNA methylation shifts when the SetDB1 enzyme is removed from mouse embryonic stem cells. SetDB1 lays down one silencing mark but also touches others, and the links between them were unclear. The team sequenced methylation across the genome over time after knockout, in two growth conditions. Nearly half of SetDB1's binding sites carried both methylation and its own mark, mostly on retrotransposons.
After knockout, both marks fell. TET enzymes stripped methylation fast at many sites, while some retrotransposons lost it slowly by dilution. Control of other marks and binding factors turned out to run on separate tracks, uncoupled from the methylation axis. Modeling pointed to rival proteins competing for distinct parts of SetDB1. The picture is of one enzyme coordinating several semi-independent silencing jobs.
Catalog record
| CREATOR | Nick G.P. Bovee, Ehsan Habibi, Zicong Liu, Stefan H.A. Hoogland, Siebren Frölich, Daniëlle Diepenbroek, Kim C.M. Bosman, Julia Arand, Irina A. Maksakova, Anouk Klein Kranenbarg, Jörn Walter, Arie B. Brinkman, Klaas W. Mulder, Joop H. Jansen, Michiel Vermeulen, Matthew Lorincz, Hendrik G. Stunnenberg |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 97 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |