Optimization of an in vitro drug screening assay for anti-Trypanosoma cruzi activity using epimastigotes
Jhindy-Tatiana Perez, María-Helena Arias, Giovanny Garavito · 2026
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Description
Jhindy-Tatiana Perez, María-Helena Arias, and Giovanny Garavito standardize a lab assay for testing drugs against the Chagas parasite, in a 2026 paper. Chagas disease leans on two old drugs with weak power and harsh side effects. Screening new compounds on the parasite's swimming epimastigote form gives useful early data, but labs each run the test differently, wrecking comparability.
The team varied the recipe systematically: growth medium, serum dose, refresh schedule, starting parasite density, and exposure time. Growth peaked on day eight. Sparse starts grew erratically, dense starts clumped, and one medium nudged parasites toward their infective shape, a confounder to avoid. Serum helped growth in proportion to dose. The tuned protocol gives screening programs a shared yardstick.
Catalog record
| CREATOR | Jhindy-Tatiana Perez, María-Helena Arias, Giovanny Garavito |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 36 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |