Single Cell Cytometry of Mouse Brain Tissue
Caroline E Roe, Chiara Miller, Nidia Calero, Jonathan M Irish, Andra L Dingman · 2026
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Description
Caroline E. Roe and four colleagues compare ways to turn adult mouse brain into a single-cell suspension suitable for mass cytometry, a method that measures dozens of markers per cell. Brain is hard to dissociate because it is densely connected and rich in debris, and different cell types survive the process unevenly.
Mechanical breakup combined with gentle enzymes such as papain or collagenase yielded roughly equal numbers of live cells from white and grey matter. A density-gradient cleanup step, by contrast, destroyed most viable cells and left too few rare cells to analyse. Cytometry identified neurons plus six major non-neuronal populations, and freezing preserved most proportions, giving other laboratories a practical preparation recipe.
Catalog record
| CREATOR | Caroline E Roe, Chiara Miller, Nidia Calero, Jonathan M Irish, Andra L Dingman |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 3 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |