Rescue a Reassortant Human-Bovine Rotavirus and a Recombinant NSP3-EGFP Rotavirus Serotype G1
Mona Aminipour, Mohsen Lotfi, Naser Harzandi, Ebrahim Babapour, Seyed Dawood Mousavi Nasab, Mostafa Ghaderi · 2026
Read Rescue a Reassortant Human-Bovine Rotavirus and a Recombinant NSP3-EGFP Rotavirus Serotype G1 by Mona Aminipour, Mohsen Lotfi, Naser Harzandi, Ebrahim Babapour, Seyed Dawood Mousavi Nasab, Mostafa Ghaderi (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13648287.txt.
Description
Mona Aminipour and five colleagues report in 2026 on engineering rotaviruses in the laboratory using reverse genetics, a method that builds viruses from plasmid DNA. Rotavirus causes severe diarrhoea in infants, and a flexible system for swapping genome segments could speed the design of new live vaccines.
Thirteen plasmids were introduced into hamster kidney cells: ten from a bovine strain, one carrying a human surface protein gene, and two helper constructs. The team rescued a mixed human-bovine virus and a second virus engineered to glow green through a fluorescent protein tag, which persisted across passages. The work is presented as proof that such vaccine candidates can be generated deliberately.
Catalog record
| CREATOR | Mona Aminipour, Mohsen Lotfi, Naser Harzandi, Ebrahim Babapour, Seyed Dawood Mousavi Nasab, Mostafa Ghaderi |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 22184 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |