The effect of rocking speed and cell inoculation density on hepatic spheroid size: A simple method for size modulation
Michael G. Megaly, Diane Tobolt, Anthony An‐Fa Dahm Chen, Bat‐Erdene Namsrai, Benjamin Fisher, Joseph Sushil Rao, Srivasupradha Ramesh, Anudari Chimedtseren, Scott L. Nyberg, Mark Clemens, Charles Y. Lee, John C. Bischof, Erik B. Finger · 2026
Read The effect of rocking speed and cell inoculation density on hepatic spheroid size: A simple method for size modulation by Michael G. Megaly, Diane Tobolt, Anthony An‐Fa Dahm Chen, Bat‐Erdene Namsrai, Benjamin Fisher, Joseph Sushil Rao, Srivasupradha Ramesh, Anudari Chimedtseren, Scott L. Nyberg, Mark Clemens, Charles Y. Lee, John C. Bischof, Erik B. Finger (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13636245.txt.
Description
Simple rocking motion can tune the size of lab-grown liver cell clusters, Megaly and colleagues show. Hepatic spheroids are small three-dimensional balls of liver cells. They work better than flat cell layers in artificial liver devices, drug-toxicity testing, and pharmacology, but many ways of making them need special equipment or static setups that resist scaling. The team grew rat liver cells on a rocker system, varying rocking speed and starting cell density, and tracked cluster shape and function over ten days.
Faster rocking made smaller clusters, with average diameter falling from about two hundred micrometres at the slowest speed to about eighty at the fastest. Function matured over time, with urea and albumin output rising across the study. Urea production peaked at a middle rocking speed. Megaly and colleagues present the rocker method as a cheap, scalable way to produce liver clusters of chosen size.
Catalog record
| CREATOR | Michael G. Megaly, Diane Tobolt, Anthony An‐Fa Dahm Chen, Bat‐Erdene Namsrai, Benjamin Fisher, Joseph Sushil Rao, Srivasupradha Ramesh, Anudari Chimedtseren, Scott L. Nyberg, Mark Clemens, Charles Y. Lee, John C. Bischof, Erik B. Finger |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 42 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |