Enrichment‐Based Duplex PCR for Early Detection of Potentially Virulent Vibrio harveyi in Aquaculture
Javier Barriga‐Cuartero, Carla Hernández‐Cabanyero, Pablo Ibanyez‐Paya, Carmen Amaro, Belen Fouz · 2026
Read Enrichment‐Based Duplex PCR for Early Detection of Potentially Virulent Vibrio harveyi in Aquaculture by Javier Barriga‐Cuartero, Carla Hernández‐Cabanyero, Pablo Ibanyez‐Paya, Carmen Amaro, Belen Fouz (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13629451.txt.
Description
Javier Barriga-Cuartero and four colleagues built a two-step test for Vibrio harveyi, a bacterium that devastates marine fish farms, which also flags isolates carrying a suspected virulence marker. Published in 2026, the workflow first grows samples briefly in a nutrient broth, then runs a duplex DNA test targeting a species gene and a plasmid gene linked to virulence.
The enrichment step improved detection roughly a thousandfold, reaching very low bacterial levels in fish tissue and water. The assay stayed specific when challenged with close Vibrio relatives and performed on spiked samples plus real material from Mediterranean farms. The authors offer it as a rapid, field-practical screen for surveillance programmes that need to spot dangerous strains early.
Catalog record
| CREATOR | Javier Barriga‐Cuartero, Carla Hernández‐Cabanyero, Pablo Ibanyez‐Paya, Carmen Amaro, Belen Fouz |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 47197 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |