Development and validation of a multiplex TaqMan real-time quantitative PCR assay for the simultaneous detection of six major bacterial respiratory pathogens in goats
Mingzhe Fu, Yi Liu, Zeyang Zhang, Weimin Li, Qi Zhang, Xingang Xu · 2026
Read Development and validation of a multiplex TaqMan real-time quantitative PCR assay for the simultaneous detection of six major bacterial respiratory pathogens in goats by Mingzhe Fu, Yi Liu, Zeyang Zhang, Weimin Li, Qi Zhang, Xingang Xu (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13626985.txt.
Description
Mingzhe Fu and five co-authors built a combined test for goat lung diseases in a 2026 paper. Breathing infections, often mixed, are a major threat to goat herds and hard to sort out one germ at a time. A single test that catches several at once speeds both treatment and surveillance.
The team built two panels of a precise gene-copying test, three germs each, covering the six main bacterial causes. Checks showed the test ignored non-target germs and could find very small amounts of each target, with steady repeat results. Two hundred nose swabs from farm goats then put the panels to real use. The detection limits and clean specificity support field use, the authors say. Such multiplex tests let vets name the culprit mix in hours instead of sending samples through slow single tests.
Catalog record
| CREATOR | Mingzhe Fu, Yi Liu, Zeyang Zhang, Weimin Li, Qi Zhang, Xingang Xu |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 39 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |