Eco-friendly spectrofluorimetric determination of fenbendazole in pharmaceutical, plasma, and milk samples using erythrosine B as a fluorescent probe

Description

Ahmad A A Mohamad and three colleagues devised a light-based test for fenbendazole, a worming drug used widely in farm and pet animals. The method leans on a pink fluorescent dye whose glow fades in exact proportion as the drug binds it. Measuring that fade gives the drug's amount in medicine samples and in spiked cow plasma and milk.

Careful checks supported a simple one-to-one binding held together by charge attraction, hydrogen bonds, and partial charge sharing, rather than fleeting collisions. The test hit accepted validation standards for accuracy, precision, and selectivity, with detection down to very small amounts. Five greenness scoring tools rated the method's footprint kindly, aside from one solvent used in plasma preparation. The authors offer it as a fast, low-cost stand-in for heavier chromatography in routine quality control.

Catalog record

Source: https://europepmc.org/article/MED/PMC13621422