R2Tg retrotransposon mediated targeted integration of exogenous genes into the 28S rDNA locus in mammalian cell lines for PKU gene therapy
Xiuyuan Wang, Ziqi Wen, Rongjun Xu, Wenjing Shi, Yiling Long, Xingri Zhan, Rui Su, Jia Fei, Yumin Li · 2026
Read R2Tg retrotransposon mediated targeted integration of exogenous genes into the 28S rDNA locus in mammalian cell lines for PKU gene therapy by Xiuyuan Wang, Ziqi Wen, Rongjun Xu, Wenjing Shi, Yiling Long, Xingri Zhan, Rui Su, Jia Fei, Yumin Li (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13621248.txt.
Description
Xiuyuan Wang and co-authors use a jumping gene tool to place a working gene into mammal cells. Their 2026 paper targets phenylketonuria, a genetic disorder where the body cannot break down one amino acid. Lasting fixes are lacking. The team used a retrotransposon from a songbird to insert copies of the missing gene into a repeated DNA zone.
Fat particles carried the tool into cells. It placed marker and target genes at the chosen site with good efficiency. Levels of the missing protein rose sharply, while the host's own nearby gene output stayed intact. The authors see the method as a precise editing route. It could one day give lifelong correction of the defect, they argue.
Catalog record
| CREATOR | Xiuyuan Wang, Ziqi Wen, Rongjun Xu, Wenjing Shi, Yiling Long, Xingri Zhan, Rui Su, Jia Fei, Yumin Li |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 14 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |