Evolutionary Dynamics-Aware De Novo Design of a Thermostable NS1-Derived Diagnostic Protein for Yellow Fever Virus
Junior Olimpio Martins, Patrícia da Silva Antunes, Rodrigo Lopes Sanz Duro, Robert Andreata-Santos, Clara Lacerda de Athayde, Gustavo Cabral-Miranda, Luiz Mário Ramos Janini, Franca Fraternali, Carlos Henrique Bezerra da Cruz, Ricardo Durães-Carvalho · 2026
Read Evolutionary Dynamics-Aware De Novo Design of a Thermostable NS1-Derived Diagnostic Protein for Yellow Fever Virus by Junior Olimpio Martins, Patrícia da Silva Antunes, Rodrigo Lopes Sanz Duro, Robert Andreata-Santos, Clara Lacerda de Athayde, Gustavo Cabral-Miranda, Luiz Mário Ramos Janini, Franca Fraternali, Carlos Henrique Bezerra da Cruz, Ricardo Durães-Carvalho (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13613397.txt.
Description
Blood tests for yellow fever often react to related viruses, which blurs diagnosis and disease tracking. Junior Olimpio Martins and co-authors map how the virus changed over time. They search its proteins for stretches that stay stable yet distinct, and settle on one piece of the NS1 protein in 2026. Guided by its structure, they design a new scaffold protein around that piece and test how it behaves. They also try computer-designed RNA aptamers as a second detection route.
The aptamers proved weak in the lab. The designed protein, though, matched commercial NS1 in their tests, stayed stable, and survived heating to 94 degrees with its shape intact after cooling. The team presents the approach as a low-cost model for other neglected viruses.
Catalog record
| CREATOR | Junior Olimpio Martins, Patrícia da Silva Antunes, Rodrigo Lopes Sanz Duro, Robert Andreata-Santos, Clara Lacerda de Athayde, Gustavo Cabral-Miranda, Luiz Mário Ramos Janini, Franca Fraternali, Carlos Henrique Bezerra da Cruz, Ricardo Durães-Carvalho |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 49 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |