Integrated in vitro transcription and oligo-dT affinity chromatography enable multi-cycle reagent recycling for mRNA manufacturing
Adithya Nair, Jixin Qu, Manoj Pohare, Thomas C. Minshull, Caroline A. Evans, Klaudia M. Slowik, Kate A. Loveday, Zoe V.R. Ashcroft, Mabrouka Maamra, Helen E. Colley, Craig Murdoch, Mark J. Dickman, Zoltán Kis · 2026
Read Integrated in vitro transcription and oligo-dT affinity chromatography enable multi-cycle reagent recycling for mRNA manufacturing by Adithya Nair, Jixin Qu, Manoj Pohare, Thomas C. Minshull, Caroline A. Evans, Klaudia M. Slowik, Kate A. Loveday, Zoe V.R. Ashcroft, Mabrouka Maamra, Helen E. Colley, Craig Murdoch, Mark J. Dickman, Zoltán Kis (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13602715.txt.
Description
Adithya Nair and eleven co-authors published this study in 2026. It links two steps of mRNA production into one recycling loop. Making mRNA medicines is costly, because the synthesis reagents are pricey and much of each batch goes to waste in cleanup.
Their process joins the synthesis reaction to the purification column through a shared buffer, so leftover reagents can go around again. Over five cycles, use of the costly cap ingredient improved more than threefold, and raw material cost efficiency more than doubled. The RNA stayed sound: its wholeness topped ninety percent, and a harmful byproduct ran far lower than in the standard process. Cell tests showed steady protein output and no creeping immune irritation across cycles. The authors present the loop as a path to cheaper mRNA at scale.
Catalog record
| CREATOR | Adithya Nair, Jixin Qu, Manoj Pohare, Thomas C. Minshull, Caroline A. Evans, Klaudia M. Slowik, Kate A. Loveday, Zoe V.R. Ashcroft, Mabrouka Maamra, Helen E. Colley, Craig Murdoch, Mark J. Dickman, Zoltán Kis |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 87 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |