Protocol for assessing protein expression levels and localization during fission yeast cytokinesis
Jack R Gregory, Yanfang Ye, Davinder Singh, Aysha H Osmani, Jian-Qiu Wu · 2026
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Description
Jack Gregory and co-authors lay out a step-by-step protocol for watching proteins as yeast cells divide, in work published in 2026. Fission yeast is a handy model for cell division, since its gear mirrors that of higher life. The protocol covers the full path from gene edits to protein counts.
Steps run from gene targeting by matched DNA swaps, through kinship tests of gene function, to sharp time-and-space microscopy. Image work runs in a free tool, and protein links are checked by pull-down tests. Protein levels are read by blot. The team notes the steps travel well to other cell zones and other model species.
Catalog record
| CREATOR | Jack R Gregory, Yanfang Ye, Davinder Singh, Aysha H Osmani, Jian-Qiu Wu |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 209 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |