Protocol for targeted analysis of oxylipins in rat plasma and mouse macrophage samples by high-resolution UHPLC-MS/MS
Larissa R. Diniz, Adriano B. Chaves-Filho, Pedro H.F. Jordão, Rosangela S. Santos, Marina T. Botana, Johan Kolmert, Ana C.A. Zucão, Marteinn T. Snaebjornsson, Eduardo M. Reis, Almut Schulze, Britta Brügger, Craig E. Wheelock, Sayuri Miyamoto · 2026
Read Protocol for targeted analysis of oxylipins in rat plasma and mouse macrophage samples by high-resolution UHPLC-MS/MS by Larissa R. Diniz, Adriano B. Chaves-Filho, Pedro H.F. Jordão, Rosangela S. Santos, Marina T. Botana, Johan Kolmert, Ana C.A. Zucão, Marteinn T. Snaebjornsson, Eduardo M. Reis, Almut Schulze, Britta Brügger, Craig E. Wheelock, Sayuri Miyamoto (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13602319.txt.
Description
Oxylipins, small signals made from fatty acids, steer inflammation and other body processes, but measuring over a hundred of them at once takes care. Larissa Diniz and co-authors publish a 2026 laboratory protocol for doing exactly that in rat blood and mouse immune cells.
The protocol walks through making standards and calibration curves, pulling the compounds out of samples, and running them through a high-resolution chromatography and mass spectrometry setup. It also covers a custom software package the team wrote for handling the resulting abundance data. The method captures 125 oxylipins in a single run. Shared protocols of this detail let other labs reproduce the measurement instead of reinventing it.
Catalog record
| CREATOR | Larissa R. Diniz, Adriano B. Chaves-Filho, Pedro H.F. Jordão, Rosangela S. Santos, Marina T. Botana, Johan Kolmert, Ana C.A. Zucão, Marteinn T. Snaebjornsson, Eduardo M. Reis, Almut Schulze, Britta Brügger, Craig E. Wheelock, Sayuri Miyamoto |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 164 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |