Rapid and efficient generation of multigene-knockout THP-1 cells using a simple CRISPR-Cas9 protocol
Chihiro Ito, Yuki Kinomura, Kensho Kawagoe, Shinobu Fujii, Toshihiro Fujii, Rikiro Fukunaga · 2026
Read Rapid and efficient generation of multigene-knockout THP-1 cells using a simple CRISPR-Cas9 protocol by Chihiro Ito, Yuki Kinomura, Kensho Kawagoe, Shinobu Fujii, Toshihiro Fujii, Rikiro Fukunaga (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13602318.txt.
Description
Knocking out genes in a floating blood cell line is a chore, since such cells take up DNA with a grudge. Chihiro Ito and co-authors give a plain route in 2026 that knocks out many genes at once. Their kit is for THP-1, a human blood cell line used in labs across the world.
Cells get a mix of guide strands for each gene, plus a dash of a drug shield strand, all sent in by a zap. A short, strong drug dose then picks the cells that took the mix up. Most genes fell in one go, and mixed cell pools could be used at once. Genes key to life need a clone step first. The route is fast and cheap to run.
Catalog record
| CREATOR | Chihiro Ito, Yuki Kinomura, Kensho Kawagoe, Shinobu Fujii, Toshihiro Fujii, Rikiro Fukunaga |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 61 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |