Protocol for cyclic DNA-damaging treatments to generate high mutational burden in the human A549 cell line
Gergely Imre, Szilvia Juhász · 2026
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Description
Gergely Imre and Szilvia Juhasz give a protocol for cyclic DNA damaging treatments that build a high mutational burden in the human A549 lung cancer cell line. Heavily mutated cell groups serve downstream genome studies. The protocol came out in twenty twenty-six.
Cells face round after round of DNA damaging agents, up to dozens of cycles. Steps cover careful dose setting so the harm stays controlled, metabolic activation of the agents, and the repeat cycle rhythm. Clone isolation follows at the end. Full use details point to a prior paper by the second author and team, the authors note.
Catalog record
| CREATOR | Gergely Imre, Szilvia Juhász |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 119 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |