A novel highly specific qPCR assay for distinguishing HBV cccDNA from rcDNA following bisulfite pretreatment
Ning Sun, Zhili Wang, Zach Heimer, Chris Wenner, Selena Y. Lin, Heidi Y. Ding, Roman Kubas, You-Yu Liu, Eliza Qiu, Hsin-Ni Liu, Hsu-Chin Hung, Wenya Huang, Tianlun Zhou, Daryl T.-Y. Lau, Ying-Hsiu Su, Haitao Guo · 2026
Read A novel highly specific qPCR assay for distinguishing HBV cccDNA from rcDNA following bisulfite pretreatment by Ning Sun, Zhili Wang, Zach Heimer, Chris Wenner, Selena Y. Lin, Heidi Y. Ding, Roman Kubas, You-Yu Liu, Eliza Qiu, Hsin-Ni Liu, Hsu-Chin Hung, Wenya Huang, Tianlun Zhou, Daryl T.-Y. Lau, Ying-Hsiu Su, Haitao Guo (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13595978.txt.
Description
A large team presents a sharper test for the hidden form of hepatitis B virus in a 2026 paper. The virus persists as a closed ring of DNA in liver cells, which blocks a full cure. Standard tests cannot tell that ring from a looser viral form, and enzyme pre steps can over or under digest.
Their test first treats the sample with a chemical that breaks the loose form's matching, then amplifies only the ring. It held up against a billion copies of the loose form and sensed as few as ten ring copies. Checks against three other methods back its accuracy. The team offers it as a standard tool for cure research.
Catalog record
| CREATOR | Ning Sun, Zhili Wang, Zach Heimer, Chris Wenner, Selena Y. Lin, Heidi Y. Ding, Roman Kubas, You-Yu Liu, Eliza Qiu, Hsin-Ni Liu, Hsu-Chin Hung, Wenya Huang, Tianlun Zhou, Daryl T.-Y. Lau, Ying-Hsiu Su, Haitao Guo |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 43 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |