Comparative oligo-FISH validates genome assemblies and delivers a precise karyotype for Lens Mill. species
Alex Junior Aparecido Silvestrini, Larissa Ramsay, Eric Bishop von Wettberg, Kirstin E Bett, Hodaka Fujii · 2026
Read Comparative oligo-FISH validates genome assemblies and delivers a precise karyotype for Lens Mill. species by Alex Junior Aparecido Silvestrini, Larissa Ramsay, Eric Bishop von Wettberg, Kirstin E Bett, Hodaka Fujii (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13592646.txt.
Description
Alex Junior Aparecido Silvestrini leads a team pinning down the true chromosome layout of lentils and their wild kin. The genus Lens holds one farmed species and six wild relatives, each with shuffled chromosome arrangements that tangle breeding and genome assembly. Gene order comparisons across species guided custom fluorescent probes, painted red and green, that light up matching stretches on real chromosomes under a microscope. Each species showed a distinct pattern.
Probe patterns for six species confirmed that assembled genomes assign chromosomes correctly and backed the rearrangements gene maps had suggested. Closest relatives stayed most alike at sequence level despite several structural swaps. Silvestrini and the team offer the probe sets as a checking tool for plant genome projects needing chromosomes they can trust.
Catalog record
| CREATOR | Alex Junior Aparecido Silvestrini, Larissa Ramsay, Eric Bishop von Wettberg, Kirstin E Bett, Hodaka Fujii |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 28 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |