Protocol for fixation and staining of Drosophila melanogaster embryos optimized for DNA-PAINT nanoscopy of adherens junction proteins
Neelima Sharma, Matthias Häring, Jennifer H Richens, Daniel St Johnston, Jörg Großhans, Deqing Kong · 2026
Read Protocol for fixation and staining of Drosophila melanogaster embryos optimized for DNA-PAINT nanoscopy of adherens junction proteins by Neelima Sharma, Matthias Häring, Jennifer H Richens, Daniel St Johnston, Jörg Großhans, Deqing Kong (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13589145.txt.
Description
Fruit fly embryos can now be prepared for a super-sharp imaging method that maps glue clusters between cells. Neelima Sharma and co-authors set out the full protocol in work from twenty twenty-six. DNA-PAINT swaps in tagged strands to reach views past the blur limit of plain scopes. The target here is the cadherin cluster that helps cells stick and keep their shape.
Steps cover embryo collection, fixing, stain work, and mounting on chambered glass slides. The end point is a sample ready for the scope. With it, labs can chart how the glue clusters sit and shift in fixed tissue at tiny scales. The protocol points readers to an earlier study for the full science behind the method.
Catalog record
| CREATOR | Neelima Sharma, Matthias Häring, Jennifer H Richens, Daniel St Johnston, Jörg Großhans, Deqing Kong |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 54 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |