Protocol for measuring endocrine-disruptive effects on transcriptional bursting using single-molecule imaging in human breast cancer cells
Pelin Yaşar, Christopher R Day, Joseph Rodriguez · 2026
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Description
Pelin Yaşar, Christopher Day, and Joseph Rodriguez publish a step-by-step protocol for watching genes fire in bursts and testing whether hormone-disrupting chemicals change the rhythm, in this 2026 paper. Genes do not transcribe steadily; they switch on in bursts, and chemicals that mimic estrogen may alter burst size or frequency.
The protocol targets TFF1, an estrogen-responsive gene in human breast cancer cells. One arm fixes cells and counts single RNA molecules at transcription sites by fluorescence tagging, across a dose range of test compounds. The other films living cells carrying a tagged TFF1 reporter, watching individual bursts in real time. Merging the two datasets resolves how a disruptor reshapes bursting dynamics. The authors detail seeding, treatment, hybridization, and imaging, and point to the companion study for full execution notes.
Catalog record
| CREATOR | Pelin Yaşar, Christopher R Day, Joseph Rodriguez |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 69 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |