From contiguity to accuracy: Validation-centered perspectives on bacterial genome assembly
Minkyung Kim, Yong-Joon Cho, Ok-Sun Kim · 2026
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Description
Minkyung Kim, Yong-Joon Cho, and Ok-Sun Kim argue that bacterial genome assemblies need checking, not just completing, in a 2026 review. Long-read sequencing now delivers near-complete or circular bacterial genomes as a matter of routine. A smooth assembly can still hide errors, from collapsed repeats and wrong joins to botched plasmid rebuilds and single-letter mistakes.
The review traces where those errors come from: repeated genome stretches, quirks of each sequencing platform, and limits of the assembly software. It then offers a decision framework that links the choice of sequencing plan, assembly route, and polishing effort to how much checking a project needs. A tiered confidence scale helps readers grade how far an assembly can be trusted for its intended use. The authors urge the field to prize accuracy and transparent reporting over completeness alone.
Catalog record
| CREATOR | Minkyung Kim, Yong-Joon Cho, Ok-Sun Kim |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 49284 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |