Metabolic Flux Analysis Reveals Cell Line‐Specific Rewiring in CHO Cells Following TCA Cycle Intermediate Feeding for Bioprocess pH Control
Sarah A. Sacco, Kevin J. Ruiz‐Márquez, Rachel Moen, Irina Trenary, Xiaolin Zhang, Xiaowen Wang, Malik Padellan, Henry Lin, Jamey D. Young · 2026
Read Metabolic Flux Analysis Reveals Cell Line‐Specific Rewiring in CHO Cells Following TCA Cycle Intermediate Feeding for Bioprocess pH Control by Sarah A. Sacco, Kevin J. Ruiz‐Márquez, Rachel Moen, Irina Trenary, Xiaolin Zhang, Xiaowen Wang, Malik Padellan, Henry Lin, Jamey D. Young (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13576813.txt.
Description
Sarah A Sacco leads a 2026 study feeding cell cultures with acids from the cell's own energy cycle to control acidity in drug-making reactors. Chinese hamster ovary cells dominate production of therapeutic proteins. Three cycle acids, malic, succinic, and alpha-ketoglutaric, replaced the usual carbon dioxide bubbling in two industrial antibody-making cell lines.
Carbon tracing showed deep rewiring of central metabolism under every acid feed, with clear differences between the two lines. Fed cultures ran the energy cycle harder, leaned less on glucose, and spilled less lactate. The alpha-ketoglutaric feed split the lines on nitrogen handling: one made more glutamine and cleared ammonia, the other stockpiled glutamate instead. Antibody output per cell rose later in the runs. The authors back acid feeding as a combined acidity and metabolism lever.
Catalog record
| CREATOR | Sarah A. Sacco, Kevin J. Ruiz‐Márquez, Rachel Moen, Irina Trenary, Xiaolin Zhang, Xiaowen Wang, Malik Padellan, Henry Lin, Jamey D. Young |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 65291 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |