Customizable host and viral transcript enrichment using CRISPR–Cas9 long-read sequencing for characterization of low-to-moderate abundance isoforms
An N T Nguyen, Jianshu Zhang, Shuxin Zhang, Miranda E Pitt, Devika Ganesamoorthy, Svenja Fritzlar, Jessie J -Y Chang, Sarah L Londrigan, Lachlan J M Coin · 2026
Read Customizable host and viral transcript enrichment using CRISPR–Cas9 long-read sequencing for characterization of low-to-moderate abundance isoforms by An N T Nguyen, Jianshu Zhang, Shuxin Zhang, Miranda E Pitt, Devika Ganesamoorthy, Svenja Fritzlar, Jessie J -Y Chang, Sarah L Londrigan, Lachlan J M Coin (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13575421.txt.
Description
Nguyen and co-authors present a new lab method in 2026 for picking out chosen RNA strands before reading them in full. Whole set RNA reading often misses strands that are present in low or middle amounts. Older ways to enrich a sample are hard to scale or cost a lot to tailor to new targets.
Their method uses the gene editing tool Cas9, guided by RNA, to cut and pull out the strands of interest. Long read machines then read those strands end to end. The team reports up to a 60 fold boost in the share of target strands. The method can be retargeted with ease to host genes or to virus genes. It also keeps sound estimates of how much of each strand was present, which makes it fit for studies of gene forms in infection and health.
Catalog record
| CREATOR | An N T Nguyen, Jianshu Zhang, Shuxin Zhang, Miranda E Pitt, Devika Ganesamoorthy, Svenja Fritzlar, Jessie J -Y Chang, Sarah L Londrigan, Lachlan J M Coin |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 33 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |