Selection and Validation of Reference Genes for RT-qPCR Analysis of Lipid Biosynthesis-Related Genes in Perilla frutescens
Siya Yue, Yuanlin Zhang, Ruiqi Tong, Haoyang Geng, Yingtao Xia, Sunyu Shi, Yueping Zheng, Zhifu Zheng, Yi Gan · 2026
Read Selection and Validation of Reference Genes for RT-qPCR Analysis of Lipid Biosynthesis-Related Genes in Perilla frutescens by Siya Yue, Yuanlin Zhang, Ruiqi Tong, Haoyang Geng, Yingtao Xia, Sunyu Shi, Yueping Zheng, Zhifu Zheng, Yi Gan (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13567130.txt.
Description
Siya Yue and co-authors hunt, in 2026, for stable reference genes in perilla, an oilseed rich in healthy fats. Gene activity studies need internal yardsticks whose levels stay steady, and perilla lacked validated ones for seed oil research. Seven housekeeping genes were tested across leaves, stems, roots, developing seeds, and drought-stressed leaves.
One gene was dropped for weak, unstable signal. Three scoring programs agreed that one ribosomal gene was steadiest overall and in seeds, while a tubulin gene led in organs and under drought stress. A pairing of the two was judged best for stressed leaves. The rankings give perilla researchers a firm base for measuring oil genes.
Catalog record
| CREATOR | Siya Yue, Yuanlin Zhang, Ruiqi Tong, Haoyang Geng, Yingtao Xia, Sunyu Shi, Yueping Zheng, Zhifu Zheng, Yi Gan |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 39 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |