Cell Disruption and RNA Extraction from Rhodococcus and Gordonia Bacteria Grown in the Presence of Hydrophobic Organic Compounds and Heavy Metal Salts
Anastasiia V. Krivoruchko, Liliia P. Komarova, Polina Y. Maltseva, Semyon M. Tyan, Anastasia A. Golysheva, Natalia A. Plotnitskaya, Maria S. Kuyukina, Irina B. Ivshina · 2026
Read Cell Disruption and RNA Extraction from Rhodococcus and Gordonia Bacteria Grown in the Presence of Hydrophobic Organic Compounds and Heavy Metal Salts by Anastasiia V. Krivoruchko, Liliia P. Komarova, Polina Y. Maltseva, Semyon M. Tyan, Anastasia A. Golysheva, Natalia A. Plotnitskaya, Maria S. Kuyukina, Irina B. Ivshina (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13565763.txt.
Description
Anastasiia Krivoruchko and co-authors present a 2026 methods paper on pulling RNA out of tough soil bacteria. The bacteria, from the genera Rhodococcus and Gordonia, help break down pollutants, but their rigid cell walls resist standard RNA extraction. That blocks gene studies of how they handle harsh chemicals.
The team tested 21 strains grown under 34 conditions, including alkanes, drugs, used frying oil, and a mercury salt. They compared three ways to break the cells open, paired with two extraction kits. Bead beating often shredded the RNA, while a strong lysozyme soak for an hour opened the cells cleanly. That route gave pure RNA in nearly every trial. They also built the first protocol for cells grown on used frying oil.
Catalog record
| CREATOR | Anastasiia V. Krivoruchko, Liliia P. Komarova, Polina Y. Maltseva, Semyon M. Tyan, Anastasia A. Golysheva, Natalia A. Plotnitskaya, Maria S. Kuyukina, Irina B. Ivshina |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 70 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |