DYNAMEX: A Time-Resolved Model for Predicting Distribution of Chemicals in In Vitro Cell Assays
Fabian C. Fischer, Liam Geyer, Miguel Rodriguez, Luise Henneberger, Beate I. Escher · 2026
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Description
A chemical's effect in a cell test depends on how much of it actually reaches the cells, and in tiny multiwell plates that amount is hard to measure. Fabian Fischer and co-authors built DYNAMEX, a time-resolved model that tracks where a chemical goes inside an assay well. The chemical may evaporate, bind to medium proteins, stick to the plastic, or enter cells, with cell growth diluting what accumulates.
The model reproduced measured uptake for ordinary compounds and for PFAS chemicals, and predicted free concentrations across 51 compounds with modest error. Simulations of 113 chemicals showed a simpler equilibrium calculation is accurate enough for most standard setups. Full kinetic modeling earns its keep when losses are heavy, serum is absent, plates are miniaturized, or cell entry is slow. The authors aim the tool at better dose estimates for safety testing.
Catalog record
| CREATOR | Fabian C. Fischer, Liam Geyer, Miguel Rodriguez, Luise Henneberger, Beate I. Escher |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 52 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |