How deep is deep enough? The importance of the detection limits of the CML minimal residual disease gDNA assay
Giovanni Micheloni, Vittoria Moretti, Annalisa Frattini, Francesco Acquati, Giorgio Binelli, Clara Belotti, Chiara Francesca Longo, Lucy Costantino, Martina Milani, Tamara Intermesoli, Rolland A. Reinbold, Giuseppe Montalbano, Orietta Spinelli, Roberto Valli, Giovanni Porta · 2026
Read How deep is deep enough? The importance of the detection limits of the CML minimal residual disease gDNA assay by Giovanni Micheloni, Vittoria Moretti, Annalisa Frattini, Francesco Acquati, Giorgio Binelli, Clara Belotti, Chiara Francesca Longo, Lucy Costantino, Martina Milani, Tamara Intermesoli, Rolland A. Reinbold, Giuseppe Montalbano, Orietta Spinelli, Roberto Valli, Giovanni Porta (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13562504.txt.
Description
Giovanni Micheloni and fourteen colleagues write to the editor about how low a test must detect leftover leukaemia before doctors can safely stop treatment. Targeted drugs have transformed chronic myeloid leukaemia care, and many patients now reach such deep responses that stopping therapy becomes a real option. Standard monitoring reads the disease signal from RNA in blood. The Varese group's letter argues that DNA-based tests, built around each patient's own chromosome break point, count leftover cells more directly and stay reliable over long follow-up.
Their study compares the two methods side by side in a monitored patient cohort, asking how detection limits differ and what that gap means for stopping decisions. Roughly half of patients who stop later relapse, so a missed trace of disease carries weight. The letter presses laboratories to state assay limits plainly, since remission-free survival may hinge on sensitivity that routine reports hide.
Catalog record
| CREATOR | Giovanni Micheloni, Vittoria Moretti, Annalisa Frattini, Francesco Acquati, Giorgio Binelli, Clara Belotti, Chiara Francesca Longo, Lucy Costantino, Martina Milani, Tamara Intermesoli, Rolland A. Reinbold, Giuseppe Montalbano, Orietta Spinelli, Roberto Valli, Giovanni Porta |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 10031 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |