SpacerScope: binary-vectorized, genome-wide off-target profiling for RNA-guided nucleases without prior candidate-site bias
Yanji Qu, Yaxuan Wang, Yan Wang, Haoru Tang, Qing Chen · 2026
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Description
Gene editing tools can cut DNA at unintended look-alike sites, and hunting those sites across a whole genome is slow, especially in huge plant genomes. Yanji Qu and co-authors present SpacerScope in this 2026 paper, a search engine that re-encodes DNA as binary vectors for very fast screening.
Tested against human lab data, the tool recovered every one of more than six thousand confirmed off-target sites. It ran over fifty times faster than a leading rival while using little memory. In an eight-copy strawberry genome it found more candidate sites than standard web tools, showing its worth for repetitive genomes.
Catalog record
| CREATOR | Yanji Qu, Yaxuan Wang, Yan Wang, Haoru Tang, Qing Chen |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 65 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |