Library-based, multiplexed strategy for mapping protein interaction networks via crosslinking
Yuxin Lu, Shuting Meng, Yi Zhang, Fei Wang, Tingting Shi, Yijia Chi, Song Chen, Dongxin Zhao · 2026
Read Library-based, multiplexed strategy for mapping protein interaction networks via crosslinking by Yuxin Lu, Shuting Meng, Yi Zhang, Fei Wang, Tingting Shi, Yijia Chi, Song Chen, Dongxin Zhao (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13551947.txt.
Description
Yuxin Lu, with seven colleagues, published a 2026 method for mapping where proteins touch. Protein contacts drive most cell work. Weak, brief contacts are easy to miss. The team built a library of variants of one proteasome protein, PSMD10. Each variant carried a light-triggered crosslinker at a new spot. Shining light froze partners in place at that spot. Mass spectrometry then named the caught partners.
Scanning many spots in one run mapped several contact zones. It also surfaced three partners not reported before. Their names are T2FA, TBA1C, and ATRIP. The team presents the library method as a broad platform. It can chart contact surfaces without prior knowledge of a protein's shape. Drug makers could use such maps to block chosen protein pairs.
Catalog record
| CREATOR | Yuxin Lu, Shuting Meng, Yi Zhang, Fei Wang, Tingting Shi, Yijia Chi, Song Chen, Dongxin Zhao |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 35026 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |