High‐Content CRISPR Screening: Methods and Applications
Yike Zhang, Yimeng Zhang, Xudong Tang, Xinyu Deng, Yitian Zhou, Yanfeng Wu, Jianhua Luo · 2026
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Description
Yike Zhang and co-authors review high-content CRISPR screening in a 2026 paper. CRISPR-Cas9 screens have become a core tool of functional genomics, knocking out genes across the genome to see which ones matter. Early screens read out little more than cell survival, enough to find essential genes and drug resistance but blind to richer behavior.
Newer high-content screens pair pooled gene edits with single-cell sequencing, detailed imaging, and spatial gene maps. Each edit can then be tied to cell state, appearance, and neighborhood, opening questions about regulation and cell diversity. The review also covers better library design, delivery methods, and analysis pipelines. Together these advances make screening results far easier to interpret and trust.
Catalog record
| CREATOR | Yike Zhang, Yimeng Zhang, Xudong Tang, Xinyu Deng, Yitian Zhou, Yanfeng Wu, Jianhua Luo |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 175 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |