An RCA-Coupled CRISPR/Cas12a Cascade-Enhanced SERS Platform for Ultrasensitive Detection of Exosomal miRNA-21 in Colorectal Cancer Serum
Jian Tao, Binbin Zeng, Caili Bi, Minyi Xu, Xiaxian Gu, Dingding Jing, Jun Sun · 2026
Read An RCA-Coupled CRISPR/Cas12a Cascade-Enhanced SERS Platform for Ultrasensitive Detection of Exosomal miRNA-21 in Colorectal Cancer Serum by Jian Tao, Binbin Zeng, Caili Bi, Minyi Xu, Xiaxian Gu, Dingding Jing, Jun Sun (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13549305.txt.
Description
Jian Tao and six co-authors wrote this 2026 study on a blood test sensitive enough to catch a whisper of colorectal cancer. Tumor cells shed tiny vesicles carrying a microRNA called miR-21, but it circulates at vanishing levels. Their platform chains three amplifiers: a reaction that copies the target into long repeating strands, a CRISPR enzyme those strands switch on, and a light-scattering readout the cutting then dims.
The cascade reached a detection limit at the femtomole level, with the whole test taking about eighty minutes. Results matched the standard laboratory method on real samples. Because the signal falls as target rises, the design turns presence into a measurable dimming. The authors pitch it for early diagnosis and for tracking the cancer over time, where catching molecular traces before symptoms return could change treatment timing.
Catalog record
| CREATOR | Jian Tao, Binbin Zeng, Caili Bi, Minyi Xu, Xiaxian Gu, Dingding Jing, Jun Sun |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 58 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |