One‐Pot RPA‐CRISPR/Cas12a Assay With Visual Readout for the Ultra‐Specific Detection of Monkeypox Virus Clade I
Boyi Li, Luyao Liu, Kaikai Jin, Zanheng Huang, Tianyi Zhang, Rong Gao, Huanxin Chen, Lijuan Niu, Changqi Fan, Haili Zhang, Pei Huang, Hualei Wang · 2026
Read One‐Pot RPA‐CRISPR/Cas12a Assay With Visual Readout for the Ultra‐Specific Detection of Monkeypox Virus Clade I by Boyi Li, Luyao Liu, Kaikai Jin, Zanheng Huang, Tianyi Zhang, Rong Gao, Huanxin Chen, Lijuan Niu, Changqi Fan, Haili Zhang, Pei Huang, Hualei Wang (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13547839.txt.
Description
Boyi Li and co-authors present a 2026 diagnostic study of a one-pot test for the more dangerous clade of monkeypox virus. Clade I spread across several countries in 2024, with death rates reaching ten percent. Field screening needs a test that is fast, exact, and workable without a full laboratory.
Their assay pairs a fast copying method with a CRISPR-based detector in a single tube, finishing in forty minutes. It reliably caught about twenty-seven copies per microliter and ignored the milder clade II and vaccinia virus entirely. It even kept working at room temperature in early checks, a boon for low-resource posts. Repeatability was tight across runs. In spiked mock samples it beat standard PCR at low concentrations. A visual readout completes the field-ready package.
Catalog record
| CREATOR | Boyi Li, Luyao Liu, Kaikai Jin, Zanheng Huang, Tianyi Zhang, Rong Gao, Huanxin Chen, Lijuan Niu, Changqi Fan, Haili Zhang, Pei Huang, Hualei Wang |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 38 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |