Protocol to identify genes required for cardiomyocyte development using Perturb-Seq
Sushama Sivakumar, Lei Wang, Huan Zhao, Mpathi Nzima, David C Park, Yihan Wang, Anjana Sundarrajan, Chikara Takeuchi, Minnie Deng, W Lee Kraus, Gary C Hon, Nikhil V Munshi · 2026
Read Protocol to identify genes required for cardiomyocyte development using Perturb-Seq by Sushama Sivakumar, Lei Wang, Huan Zhao, Mpathi Nzima, David C Park, Yihan Wang, Anjana Sundarrajan, Chikara Takeuchi, Minnie Deng, W Lee Kraus, Gary C Hon, Nikhil V Munshi (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13543896.txt.
Description
Sushama Sivakumar and colleagues published a screening protocol in two thousand twenty-six. Their method finds genes needed for heart muscle development. It combines gene editing screens with single-cell gene reading. Large screens cost a lot, though. Developing cells also vary, which complicates analysis. The protocol walks through each step carefully.
Steps cover guide library building and cell infection. Heart differentiation comes next, then cell tagging. Heavy loading boosts how many cells get read. Sequencing and data analysis close the pipeline. Other labs can repeat the method exactly. Developmental biologists gain a ready recipe for gene hunts. Heart formation genes remain its proving ground.
Catalog record
| CREATOR | Sushama Sivakumar, Lei Wang, Huan Zhao, Mpathi Nzima, David C Park, Yihan Wang, Anjana Sundarrajan, Chikara Takeuchi, Minnie Deng, W Lee Kraus, Gary C Hon, Nikhil V Munshi |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 90 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |