CRISPRessoSea: streamlined analysis and comparison of pooled amplicon CRISPR screens
Samuel Coleman, Jacob Tye, Daxton Furniss, Robert Sainsbury, Abhay Rastogi, Xincen Xi, Balazs Murnyak, Jason Bell, Joseph Skeate, Minjing Wang, Beau Webber, Branden Moriarity, Kendell Clement · 2026
Read CRISPRessoSea: streamlined analysis and comparison of pooled amplicon CRISPR screens by Samuel Coleman, Jacob Tye, Daxton Furniss, Robert Sainsbury, Abhay Rastogi, Xincen Xi, Balazs Murnyak, Jason Bell, Joseph Skeate, Minjing Wang, Beau Webber, Branden Moriarity, Kendell Clement (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13543416.txt.
Description
Samuel Coleman and colleagues published a 2026 software paper presenting CRISPRessoSea, a tool for analysing pooled CRISPR editing experiments. CRISPR editing can also cut at unintended sites resembling the target. Pooled amplicon sequencing, which reads many edited regions at once, is a standard way to check both intended and off-target changes. Comparing results across many samples and experiments, however, has lacked a standard workflow.
The new package processes, compares, and visualises editing rates from such pooled experiments in a uniform way. It covers both classic nuclease editing and base editing, where single letters are changed without cutting both strands. Outputs are standardised, data-rich summaries meant to make editing specificity easier to judge and reproduce across laboratories. The software is freely available, and the authors position it as shared infrastructure for a field where transparent off-target reporting matters for clinical translation.
Catalog record
| CREATOR | Samuel Coleman, Jacob Tye, Daxton Furniss, Robert Sainsbury, Abhay Rastogi, Xincen Xi, Balazs Murnyak, Jason Bell, Joseph Skeate, Minjing Wang, Beau Webber, Branden Moriarity, Kendell Clement |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 27731 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |