Establishment of a CRISPR‐Cas9 Library for Indica Rice and Identification of OsOPR5 (LOC_Os06g11210) as a Regulator of Root Architecture

Sankhadeep Chowdhury, Suprava Priyadarsini Nayak, Rajashree Pattanayak, Shaswati Sardar, Ashis Majhi, Banita Yadav, Abhilash Dasari, Rushil Mandlik, Humira Sonah, Rupesh Deshmukh, Ishaan Gupta, Petra Bauer, Hasthi Ram · 2026

Read Establishment of a CRISPR‐Cas9 Library for Indica Rice and Identification of OsOPR5 (LOC_Os06g11210) as a Regulator of Root Architecture by Sankhadeep Chowdhury, Suprava Priyadarsini Nayak, Rajashree Pattanayak, Shaswati Sardar, Ashis Majhi, Banita Yadav, Abhilash Dasari, Rushil Mandlik, Humira Sonah, Rupesh Deshmukh, Ishaan Gupta, Petra Bauer, Hasthi Ram (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13543039.txt.

Description

Sankhadeep Chowdhury and co-authors build a gene-editing library for indica rice in a 2026 study. Rice genome data abound, yet the roles of most genes stay unknown. Editing libraries can test genes at scale, but indica varieties resist the lab steps involved.

Their library targets twelve thousand genes in a widely grown indica cultivar. Checks showed high guide coverage and accuracy above eighty percent. Nine in ten edited plants carried a change at the intended site. One mutant revealed a gene that boosts root growth: plants lacking it grew fewer, shorter roots. Hormone levels stayed normal, hinting at a specialized role. The platform opens indica rice to systematic gene hunts.

Catalog record

CREATORSankhadeep Chowdhury, Suprava Priyadarsini Nayak, Rajashree Pattanayak, Shaswati Sardar, Ashis Majhi, Banita Yadav, Abhilash Dasari, Rushil Mandlik, Humira Sonah, Rupesh Deshmukh, Ishaan Gupta, Petra Bauer, Hasthi Ram
YEAR2026
COLLECTIONPAPERS
FORMATTXT
FILE SIZE34 KB
VERSION1.0
LICENSECC BY 4.0
RIGHTSCC BY
SOURCEEurope PMC

Source: https://europepmc.org/articles/PMC13543039

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