Measuring cytokines in quarter milk samples with a fluorescent bead-based multiplex assay
Anja Sipka, Matthias Wieland · 2026
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Description
Anja Sipka and Matthias Wieland published a 2026 study testing a fluorescent bead-based multiplex assay, a laboratory method that measures several substances in one small sample, on milk from individual udder quarters. They targeted three cytokines, the signalling proteins IL-10, TNF-alpha, and IFN-gamma, which rise or fall with udder inflammation. Samples came from quarters infected with major mastitis pathogens, from mastitic quarters where culture grew nothing, and from healthy quarters.
Cytokine levels differed between the culture outcome groups, hinting that these proteins carry information about infection beyond what bacterial culture gives. Variation between individual samples was nevertheless high, which limits how sharply the markers separate one quarter from another. The authors conclude the assay works for measuring these cytokines in quarter milk and could support high-throughput screening for mastitis biomarkers, a faster complement to waiting for culture results.
Catalog record
| CREATOR | Anja Sipka, Matthias Wieland |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 24547 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |