Multi-omics reveal molecular changes during suspension adaptation of HEK293 cells
Benyao Zhang, Shishi Li, Jingjing Liu, Wenhao Su, Xiaohuan Zhang, Xiuxiu Ren, Tingting Zhao, Qiufang Huang, Zihao Ge, Jiangbo Wei · 2026
Read Multi-omics reveal molecular changes during suspension adaptation of HEK293 cells by Benyao Zhang, Shishi Li, Jingjing Liu, Wenhao Su, Xiaohuan Zhang, Xiuxiu Ren, Tingting Zhao, Qiufang Huang, Zihao Ge, Jiangbo Wei (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13542038.txt.
Description
Benyao Zhang and nine colleagues chart, in a 2026 multi-omics study, what changes inside human kidney 293 cells as they learn to live floating in suspension instead of stuck to a surface. Industry grows these cells in suspension to manufacture viral vectors, yet the adaptation journey was poorly described.
Adapted cells grew more slowly, drank less glucose, made more lactate, and gripped surfaces more weakly. Gene, protein, and metabolite profiling together found thousands of shifted genes and hundreds of altered metabolites. The pattern resolved into three survival moves: rebuilding internal structure, rewiring energy metabolism, and hardening stress defences. The resulting line outproduced an internal reference for adenoviral vector work.
Catalog record
| CREATOR | Benyao Zhang, Shishi Li, Jingjing Liu, Wenhao Su, Xiaohuan Zhang, Xiuxiu Ren, Tingting Zhao, Qiufang Huang, Zihao Ge, Jiangbo Wei |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 86901 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |