Applications of transposon-insertion sequencing for understanding bacterial physiology
Emily C. A. Goodall, Stineke van Houte · 2026
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Description
Emily Goodall and Stineke van Houte review a genome-wide method for probing bacteria in a 2026 paper. The method, transposon-insertion sequencing, peppers a bacterium's genome with jumping genes, then sequences where they landed in thousands of mutants at once. A gene that never takes a hit is one the cell cannot live without. That alone made the method famous.
The review ranges wider. The same trick can assign jobs to the many bacterial genes whose function is still unknown, a list that grows with every new genome. It can also map which genes matter under stress, in hosts, or against drugs. The method now works in more than one hundred species. The authors close with where it may go next, from finer maps to new kinds of screens.
Catalog record
| CREATOR | Emily C. A. Goodall, Stineke van Houte |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 31 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |