LC–MS assay for quantifying ornithine decarboxylase activity in the biological matrix and cultured cells using stable isotope‑labeled ornithine
Yuma Shiomi, Naoya Ogawa, Kentaro Takahama, Atsushi Murai, Kyohei Furukawa · 2026
Read LC–MS assay for quantifying ornithine decarboxylase activity in the biological matrix and cultured cells using stable isotope‑labeled ornithine by Yuma Shiomi, Naoya Ogawa, Kentaro Takahama, Atsushi Murai, Kyohei Furukawa (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13534377.txt.
Description
Yuma Shiomi and colleagues present in 2026 a non-radioactive lab method to measure activity of ornithine decarboxylase, the rate-limiting enzyme in polyamine production. Polyamines support cell growth and must stay tightly controlled, yet older assays need radioactive materials and special facilities. Their method feeds cells a labelled form of ornithine instead.
Labelled product was measured by liquid chromatography with mass checks, with clear peaks in test samples and near-zero signal in controls. Tweaks to time and dose kept results linear and precise, and a known blocker lowered activity as expected. Labs gain a safer tool for polyamine research.
Catalog record
| CREATOR | Yuma Shiomi, Naoya Ogawa, Kentaro Takahama, Atsushi Murai, Kyohei Furukawa |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 38960 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |