The diagnostic potential of combined quantitative polymerase chain reaction and next-generation sequencing using the same primers for periprosthetic joint infection
Narumi Ueda, Jun Inoue, Kazuyuki Okuda, Hirokazu Iida · 2026
Read The diagnostic potential of combined quantitative polymerase chain reaction and next-generation sequencing using the same primers for periprosthetic joint infection by Narumi Ueda, Jun Inoue, Kazuyuki Okuda, Hirokazu Iida (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13532281.txt.
Description
Narumi Ueda, Jun Inoue, and Kazuyuki Okuda combine in 2026 two gene methods to diagnose prosthetic joint infection more reliably. Broad bacterial gene screening first measures how much bacterial material a sample holds, then sequencing names the germs only when levels pass a safe threshold. Samples came from loosened joint fluid and surgical tissue.
Screening agreed with itself almost perfectly and matched culture results with fair sensitivity and specificity. Sequencing confirmed most culture-positive infections and caught three culture-negative ones, including a case whose flagged germ caused a real infection two years later. Threshold-first testing filters contamination noise.
Catalog record
| CREATOR | Narumi Ueda, Jun Inoue, Kazuyuki Okuda, Hirokazu Iida |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 74 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |