Sequence‐Specific Primer Polymerase Chain Reaction Genotyping of the Human IL33 Polymorphism rs1929992 (T > C) with Sanger Sequencing Validation
Aléia Harumi Uchibaba Yamanaka, Guilherme Castellani, Matheus Braga, Josiane Bazzo de Alencar, Letícia Cristina de Almeida Silva, Quirino Alves de Lima Neto · 2026
Read Sequence‐Specific Primer Polymerase Chain Reaction Genotyping of the Human IL33 Polymorphism rs1929992 (T > C) with Sanger Sequencing Validation by Aléia Harumi Uchibaba Yamanaka, Guilherme Castellani, Matheus Braga, Josiane Bazzo de Alencar, Letícia Cristina de Almeida Silva, Quirino Alves de Lima Neto (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13528966.txt.
Description
Aléia Yamanaka and co-authors present a 2026 lab protocol for typing a gene variant tied to immune response. The variant sits in IL33, a signal gene for inflammation and tissue repair, and one letter form makes more of the signal than the other. The method uses matched primers that grow only the letter form they target, run in twin reactions. A housekeeping gene serves as the built-in check, and band patterns on a gel give the call.
Settings for heat, salts, primer balance, and DNA dose were tuned for clean, repeat runs. Calls were checked against direct gene reading and held up. A cheap, plain method suits labs without high-end gear. Variant maps feed studies of allergy and immune disease risk.
Catalog record
| CREATOR | Aléia Harumi Uchibaba Yamanaka, Guilherme Castellani, Matheus Braga, Josiane Bazzo de Alencar, Letícia Cristina de Almeida Silva, Quirino Alves de Lima Neto |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 26 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |