A Practical Gut‐On‐Chip Workflow for Imaging‐Based Assessment of Epithelial Barrier Dynamics During E. coli Co‐Culture
Shih‐Wei Chiang, Chuan‐Yi Yang, Guan‐Yu Chen · 2026
Read A Practical Gut‐On‐Chip Workflow for Imaging‐Based Assessment of Epithelial Barrier Dynamics During E. coli Co‐Culture by Shih‐Wei Chiang, Chuan‐Yi Yang, Guan‐Yu Chen (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13521455.txt.
Description
Shih-Wei Chiang, Chuan-Yi Yang and Guan-Yu Chen present in 2026 a practical gut-on-chip routine for filming how the intestinal barrier reacts while living E. coli bacteria share the channel. Flat static cultures lack flow forces, while animal work resists detailed live imaging.
A commercial two-channel chip held human gut cells under gentle flow, forming a sealed, polarised layer with continuous junctions and a mucus coat. Labelled bacteria were then added at varied flow rates. Slow flow allowed bacterial overgrowth that thinned and detached the layer within four days, while faster tolerated flow protected structure. Settings guidance should help labs run reproducible co-cultures.
Catalog record
| CREATOR | Shih‐Wei Chiang, Chuan‐Yi Yang, Guan‐Yu Chen |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 39837 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |