A high-throughput co-culture CRISPR array for rapid fitness hypothesis testing and growth engineering in CHO cells
Jannis Peter Marzluf, Lea Huckfeldt, Dominik Haidas, Christoph Zehe, Ann-Cathrin Leroux · 2026
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Description
SummaryCell growth and fitness shape mammalian biomanufacturing performance, but subtle genetic effects are difficult to resolve with conventional growth assays. We developed a high-throughput competitive co-culture CRISPR array in Chinese hamster ovary (CHO) suspension cells that pairs a CRISPR-competent population with a CRISPR-inactive green fluorescent protein (GFP) reference pool and tracks GFP-ratio dynamics by flow cytometry.
Catalog record
| CREATOR | Jannis Peter Marzluf, Lea Huckfeldt, Dominik Haidas, Christoph Zehe, Ann-Cathrin Leroux |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 123 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |