Quantitative MALDI-MSI Workflow for Lipids with a LC-MS-Based Quality Control Pipeline
Felix-Levin Hormann, Sven Heiles · 2026
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Description
Hormann and Heiles build a workflow for measuring how much of each fat molecule sits in each spot of a tissue slice. Imaging methods map where lipids are but rarely how much, while ordinary liquid chromatography measures amounts precisely yet loses all spatial information. Their 2026 workflow sprays thirteen isotope-labelled lipid standards onto mouse heart tissue. It images everything with a laser-based technique, then checks the standards and identifications with the chromatography method as quality control.
The sprayed standards landed evenly. The comparison also exposed a quirk: imaging variability depended on both a lipid's amount and its chemical class, unlike the chromatography method where only scarcity hurt. Polar lipids matched well between the two methods, while oily triglycerides overlapped poorly. The authors conclude the control pipeline is not optional decoration but what makes the imaging numbers trustworthy.
Catalog record
| CREATOR | Felix-Levin Hormann, Sven Heiles |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 45 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |